Application

Anti-Human IgG (whole molecule) antibody has been used inenzyme linked immunosorbent assay (ELISA)printing and processing of reverse phase protein microarrayswestern blotradial immunodiffusion

Biochem/physiol Actions

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab fragments or bivalent F(ab′)₂ fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IgGis present in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins.IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates.It consists of four sub-classesi.eIgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γchains)and two light chains (κ or λchains)which are linked by inter-chaindisulfidebonds.The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have oneN-terminal variable domain (VL) andoneconstant domain(CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding).The antigen binds to the V regions of theantibody.

Physical form

Anti-Human IgG is supplied as a liquid containing 15 mM sodium azide as a preservative

Storage and Stability

For continuous use, store at2-8 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.